Diet of Long-Eared Owl and Common Kestrel in an Urban Landscape (Ukraine)

In the present study we performed a comparative dietary analysis of two predatory birds, the Long-eared Owl (Asio otus) and the Common Kestrel (Falco tinnunculus) in the district of Lviv city. We found that the Long-eared Owl and the Common Kestrel are typical small mammal specialists within the urban ecosystem. Considering the abundance and biomass of prey, small mammals comprise 98.4% of the Long-eared Owl’s diet. The species composition of mammals coincides almost 50% in the food intake comparison of the two birds. It has been established that the main prey of both species is the Common Vole (Microtus arvalis). The diet of the Common Kestrel is more varied, compared to the Long-eared Owl, due to the consumption of different species of insects (families Gryllotalpidae, Tettigoniidae, Carabidae and Scarabaeidae), reptiles and birds. This result suggested that dietary plasticity of the Common Kestrel facilitate successful adaptation to the urban landscape. The Long-eared Owl is more narrowly specialized in feeding on murine rodents, which reduces the trophic competition between the two predatory birds and allows the coexistence of two predators in the urban ecosystem

Phylogeographic history of grey wolves in Europe

Background: While it is generally accepted that patterns of intra-specific genetic differentiation are substantially affected by glacial history, population genetic processes occurring during Pleistocene glaciations are still poorly understood. In this study, we address the question of the genetic consequences of Pleistocene glaciations for European grey wolves. Combining our data with data from published studies, we analysed phylogenetic relationships and geographic distribution of mitochondrial DNA haplotypes for 947 contemporary European wolves. We also compared the contemporary wolf sequences with published sequences of 24 ancient European wolves. Results: We found that haplotypes representing two haplogroups, 1 and 2, overlap geographically, but substantially differ in frequency between populations from south-western and eastern Europe. A comparison between haplotypes from Europe and other continents showed that both haplogroups are spread throughout Eurasia, while only haplogroup 1 occurs in contemporary North American wolves. All ancient wolf samples from western Europe that dated from between 44,000 and 1,200 years B.P. belonged to haplogroup 2, suggesting the long-term predominance of this haplogroup in this region. Moreover, a comparison of current and past frequencies and distributions of the two haplogroups in Europe suggested that haplogroup 2 became outnumbered by haplogroup 1 during the last several thousand years. Conclusions: Parallel haplogroup replacement, with haplogroup 2 being totally replaced by haplogroup 1, has been reported for North American grey wolves. Taking into account the similarity of diets reported for the late Pleistocene wolves from Europe and North America, the correspondence between these haplogroup frequency changes may suggest that they were associated with ecological changes occurring after the Last Glacial Maximum

Wolf outside, dog inside? The genomic make-up of the Czechoslovakian Wolfdog

Background Genomic methods can provide extraordinary tools to explore the genetic background of wild species and domestic breeds, optimize breeding practices, monitor and limit the spread of recessive diseases, and discourage illegal crossings. In this study we analysed a panel of 170k Single Nucleotide Polymorphisms with a combination of multivariate, Bayesian and outlier gene approaches to examine the genome-wide diversity and inbreeding levels in a recent wolf x dog cross-breed, the Czechoslovakian Wolfdog, which is becoming increasingly popular across Europe. Results Pairwise FST values, multivariate and assignment procedures indicated that the Czechoslovakian Wolfdog was significantly differentiated from all the other analysed breeds and also well-distinguished from both parental populations (Carpathian wolves and German Shepherds). Coherently with the low number of founders involved in the breed selection, the individual inbreeding levels calculated from homozygosity regions were relatively high and comparable with those derived from the pedigree data. In contrast, the coefficient of relatedness between individuals estimated from the pedigrees often underestimated the identity-by-descent scores determined using genetic profiles. The timing of the admixture and the effective population size trends estimated from the LD patterns reflected the documented history of the breed. Ancestry reconstruction methods identified more than 300 genes with excess of wolf ancestry compared to random expectations, mainly related to key morphological features, and more than 2000 genes with excess of dog ancestry, playing important roles in lipid metabolism, in the regulation of circadian rhythms, in learning and memory processes, and in sociability, such as the COMT gene, which has been described as a candidate gene for the latter trait in dogs. Conclusions In this study we successfully applied genome-wide procedures to reconstruct the history of the Czechoslovakian Wolfdog, assess individual wolf ancestry proportions and, thanks to the availability of a well-annotated reference genome, identify possible candidate genes for wolf-like and dog-like phenotypic traits typical of this breed, including commonly inherited disorders. Moreover, through the identification of ancestry-informative markers, these genomic approaches could provide tools for forensic applications to unmask illegal crossings with wolves and uncontrolled trades of recent and undeclared wolfdog hybrids

North-south differentiation and a region of high diversity in European wolves (Canis lupus)

European wolves (Canis lupus) show population genetic structure in the absence of geographic barriers, and across relatively short distances for this highly mobile species. Additional information on the location of and divergence between population clusters is required, particularly because wolves are currently recolonizing parts of Europe. We evaluated genetic structure in 177 wolves from 11 countries using over 67K single nucleotide polymorphism (SNP) loci. The results supported previous findings of an isolated Italian population with lower genetic diversity than that observed across other areas of Europe. Wolves from the remaining countries were primarily structured in a north-south axis, with Croatia, Bulgaria, and Greece (Dinaric-Balkan) differentiated from northcentral wolves that included individuals from Finland, Latvia, Belarus, Poland and Russia. Carpathian Mountain wolves in central Europe had genotypes intermediate between those identified in northcentral Europe and the Dinaric-Balkan cluster. Overall, individual genotypes from northcentral Europe suggested high levels of admixture. We observed high diversity within Belarus, with wolves from western and northern Belarus representing the two most differentiated groups within northcentral Europe. Our results support the presence of at least three major clusters (Italy, Carpathians, Dinaric-Balkan) in southern and central Europe. Individuals from Croatia also appeared differentiated from wolves in Greece and Bulgaria. Expansion from glacial refugia, adaptation to local environments, and human-related factors such as landscape fragmentation and frequent killing of wolves in some areas may have contributed to the observed patterns. Our findings can help inform conservation management of these apex predators and the ecosystems of which they are part

Seasonal features of the diet of predatory mammals in the western regions of Ukraine

The article analyses feeding specifics depending on the season of five species of predatory mammals belonging to two families: Mustelidae and Canidae. It was established that the largest number of food objects in the red fox’s diet is characteristic for the winter period (44 objects). In winter, this species enriches its diet with invertebrates, fish, domestic mammals, and ungulates in small quantities. The main objects of food during this period are rodents (19%) and birds (16.6%). Rodents are also the basis of the weasel’s winter diet (50%). Both species of marten also consume rodents and birds in the winter, however, the pine marten hunts birds more often (17.4%) compared to rodents (13%), whereas the stone marten rarely hunts birds in the winter and the basis of its diet is made up by rodents (23.1%). The spring period is characterised by a sharp decrease in objects of plant origin in the diet of all carnivorous mammals considered. In spring, the stone marten consumes the largest share of animal feed (80%). The share of animals in the pine marten’s diet also increases, but this species actively hunts invertebrates (37.1%). Birds were not detected in the diet of the pine marten in spring, but they are present in the diet of the weasel in this period (5.9%). Almost half of the summer diet of all species considered consists of plant objects. All predators are characterised by the consumption of a large number of invertebrates during this period, with the lowest share of them in the diet of the stone marten (5.6%), whose main food in the summer is plants (55.6%), rodents (22.2%) and birds (16.7%). In autumn, the share of plant components in the diet of predators is more than 50%, and this period is characterised by having the highest share of juicy fruits in the diet throughout the year, except for the weasel, in the diet of which plants are rarely found during this period (21.4%). The basis of the diet of this species during this period is mammals (64.3%). The share of rodents (15.5%) and birds (8.3%) in the red fox’s diet increases in the autumn period. Inedible objects were recorded in food samples of two species: red fox and pine marten. For the marten, the largest number of these objects was registered in the summer period, and for the fox, non-food objects were absent only in the summer diet

Phylogeographic history of grey wolves in Europe

Abstract Background While it is generally accepted that patterns of intra-specific genetic differentiation are substantially affected by glacial history, population genetic processes occurring during Pleistocene glaciations are still poorly understood. In this study, we address the question of the genetic consequences of Pleistocene glaciations for European grey wolves. Combining our data with data from published studies, we analysed phylogenetic relationships and geographic distribution of mitochondrial DNA haplotypes for 947 contemporary European wolves. We also compared the contemporary wolf sequences with published sequences of 24 ancient European wolves. Results We found that haplotypes representing two haplogroups, 1 and 2, overlap geographically, but substantially differ in frequency between populations from south-western and eastern Europe. A comparison between haplotypes from Europe and other continents showed that both haplogroups are spread throughout Eurasia, while only haplogroup 1 occurs in contemporary North American wolves. All ancient wolf samples from western Europe that dated from between 44,000 and 1,200 years B.P. belonged to haplogroup 2, suggesting the long-term predominance of this haplogroup in this region. Moreover, a comparison of current and past frequencies and distributions of the two haplogroups in Europe suggested that haplogroup 2 became outnumbered by haplogroup 1 during the last several thousand years. Conclusions Parallel haplogroup replacement, with haplogroup 2 being totally replaced by haplogroup 1, has been reported for North American grey wolves. Taking into account the similarity of diets reported for the late Pleistocene wolves from Europe and North America, the correspondence between these haplogroup frequency changes may suggest that they were associated with ecological changes occurring after the Last Glacial Maximum.</p

The use of museum skins for genomic analyses of temporal genetic diversity in wild species

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First insights into the molecular population structure and origins of the invasive Chinese sleeper, Perccottus glenii, in Europe

The aim of our study was to provide a first overview of the population genetic structure of the invasive Chinese sleeper, Perccottus glenii, (Actinopterygii: Odontobutidae) in European water bodies. This species originates from inland waters of north-eastern China, northern North Korea and the Russian Far East. The 1172 bp long portion of the cytochrome b gene was sequenced from Chinese sleeper specimens collected from a variety of water bodies in Belarus, Bulgaria, Hungary, Germany, Latvia, Lithuania, Poland, Russia (European part) and Ukraine. Our study revealed that the invasive Chinese sleeper in Europe consists of at least three distinct haplogroups that may represent independent introduction events from different parts of its native area; i.e. three founding populations: (1) Baltic haplogroup that may originate either from fish introduced inadvertent from Russia or from some unidentified source (release by aquarists). So far, this haplogroup has been found only in the Daugava basin in Latvia. (2) East-European haplogroup that may originate from an unintentional introduction to the Volga basin in Russia and has expanded westward. So far, this group was recorded in the Volga, Upper Dnieper and Neman drainages in Belarus, Lithuania, and Russia. (3) Carpathian haplogroup, that originated from individuals unintentionally introduced with Asian cyprinid fishes to Lviv region in Ukraine and are now widely distributed in Central Europe

Additional file 4: of Wolf outside, dog inside? The genomic make-up of the Czechoslovakian Wolfdog

Figure S4. Comparison between the individual frequency of ROHs (FROH), calculated in SVS as the proportion of ROHs on the genome length spanned by the analysed SNPs (on the horizontal axis), and the individual Wright’s inbreeding coefficient (COI), estimated from the pedigrees with the software U-WGI (on the vertical axis). The two inbreeding indexes are significantly (p < 0.01) correlated. (PDF 71 kb